Isolation of single yeast cells by optical trapping

Individual yeast cells can be successfully isolated and recultured on plates with a new isolation method making use of optical trapping with infrared laser light. The cells can be selected on morphological criteria by high resolution microscopy. The isolation device is constructed from two coverslips separated by spacers, in which selected cells are transferred to a plastic capillary, using the optical trap. To test the procedure, selection experiments were done with a mixture of two Saccharomyces cerevisiae strains, distinguishable both in fluorescence microscopy and on agar plates. These experiments showed that only selected cells were isolated, and close to 100% of the isolated stationary-phase cells formed colonies on agar plates, indicating a high recovery. A lower recovery was obtained with exponential-phase cells, possibly because of a higher sensitivity to laser irradiation. Applications for this method may include the isolation of mutants with altered morphology and the isolation of subpopulations of yeast cultures, for their separate investigation or for the initiation of pure cultures.     

Stress concentration and fatigue of profiled reinforcing steels

Stress concentrations arise from profiles of ribbed reinforcing steel bars and in this study the results of calculated stress concentration factors (SCF), by using finite element method, are related to the fatigue test results. It is apparent that the degree of confinement of a ribbed bar embedded in concrete not only affects the magnitude of the ultimate bond stress but also the bond stress-slip relation. Thus the rib geometry or profile pattern is designed for optimum bond characteristics and not for fatigue considerations. However the rib geometry influences the fatigue performance through the SCFs arising from the root radius, width and flank angles of the profiles. It is shown that these latter factors have a significant influence on the fatigue behaviour of reinforcing steel bars.     

筒形件反旋工艺塑性流动刚塑性有限元分析

建立了筒形件反旋工艺平面变形刚塑性有限元分析的力学模型，比较了把反旋工艺视为正挤和本文力学模型条件下的速度场，有限元分析结果与反旋实际条件相符。     

A calibration method for the energy-integrating function in photovoltaic system monitoring

Solar radiation has an irregularly varying factor due to a basic day and night cycle and climatic conditions. For such conditions a data sampling interval is important to ensure the accuracy of energy monitoring in a photovoltaic system. While treating a system monitoring equipment as a black box. the author has developed the method of calibrating an energy-integrating function. At first for various input waveforms, the relationship between sampling intervals and quasi-integration outputs have been examined by trapezoidal rule. In the numerical simulation the phases of the sampling clock also are considered Then it is concluded that a sampling interval can be inspected through outside observation only by using a rectangular single pulse. By applying the pulse to the energy-integrating process, two kinds of integrated outputs can be obtained for different sampling phases. The calculated difference between both outputs can uniquely give the sampling interval being inspected. Conditions to ensure measuring accuracy are discussed and the validity of this method has been demonstrated experimentally. Practical calibrating procedures also are proposed for the integrating function of PV system monitoring.     

加速SPICE进行三维电磁分析

互连结构的电磁分析越来越受到人们的重视.针对三维互连,A.E.Ruehli提出了部分元等效电路法.但该法生成的等效电路具有紧耦合性,用SPICE进行分析时稀疏矩阵技术已失去原先的优越性.本文采用广义残量法作为大型紧耦合线性方程组的求解工具以取代SPICE中的LU分解法,并辅以初值预估.实际计算表明,本文的方法提高了运算速度.广义残量法也可用于矩量法的方程求解中.     

用频谱分析法对地下水位进行中长期预报

本文把影响地下水位变化过程作为随机过程，用频谱分析法建立随机水动力学模型模拟地下水位的变化过程，并将其用于地下水位的中长期预报。通过实例验证它具有较高的精度。     

低空大气对连续波干涉仪系统相位稳定性的影响

针对三站连续波干涉仪系统，根据实时测得的系统场区大气折射率漂移数据，应用几何光学方法，从理论上估计了连续波干波仪系统两条微波基线传输的相位稳定性。     

基于Matlab的线性规划问题求解

线性规划是运筹学中研究较早、发展较快、应用广泛、方法成熟的一个重要分支,它是辅助人们进行科学管理的一种重要的数学方法.文章首先介绍了线性规划的基本概念及标准形式,着重讨论了线性规划问题的三种常用解法:单纯形法、直接搜索法以及遗传算法,最后在Matlab R2009a环境下进行了仿真.通过结果可以看出,用Matlab求解线性规划问题,可以避免手工的烦琐计算,大大地提高工作效率和结果的准确性.     

基于C#语言的图片插入方法比较

图片是传递与表达信息的重要形式,由于图片数据特殊编码方式,它的存储和显示显得比较复杂。C#功能强大,使用方便,已经成为一种主流的计算机软件编程语言。文章介绍了使用C#向数据库中插入图片的方法,并加以比较。     

光时域反射计的设计与实现

文章介绍了一种光时域反射计的设计和实现,详细介绍了各个硬件模块和使用FPGA实现数据等效采样和消噪处理的方法.模块配备LAN和RS232接口,方便集成以及分布式部署,利于推广应用,从而带来较好的经济、社会和管理效益.